| Endocrinology B317 Lab, hCG Binding to Tissues, Absorbance Readings at 450 nm | |||||||||||||
| Tissue (series) x Time (min) | |||||||||||||
| Group | A0 | A5 | A20 | A75 | B0 | B5 | B20 | B75 | C0 | C5 | C20 | C75 | [hCG] (ng/mL) |
| 1 | 0.280 | 0.422 | 0.383 | 0.465 | 0.369 | 0.416 | 0.458 | 2.700 | 4.000 | 4.000 | 4.000 | 4.000 | 0 |
| 7 | 0.329 | 0.398 | 0.455 | 0.443 | 0.443 | 0.544 | 0.451 | 0.487 | 4.000 | 3.971 | 2.729 | 4.000 | 0 |
| 2 | 0.386 | 0.406 | 0.600 | 0.755 | 0.374 | 2.787 | 0.572 | 0.736 | 3.804 | 3.688 | 4.000 | 3.258 | 0.04 |
| 3 | 4.000 | 4.000 | 0.939 | 2.009 | 2.500 | 4.000 | 3.090 | 4.000 | 4.000 | 3.465 | 4.000 | 4.000 | 0.4 |
| 4 | 0.388 | 0.405 | 0.474 | 1.002 | 0.337 | 0.449 | 0.570 | 0.720 | 3.157 | 2.750 | 3.830 | 3.243 | 4 |
| 5 | 0.442 | 0.822 | 1.032 | 1.872 | 0.374 | 0.563 | 0.982 | 1.236 | 3.118 | 3.778 | 4.000 | 3.907 | 40 |
| 8 | 0.625 | 1.035 | 1.404 | 1.703 | 0.692 | 0.694 | 1.155 | 1.487 | 4.000 | 3.646 | 2.853 | 3.149 | 40 |
| 6 | 1.263 | 0.497 | 0.751 | 1.007 | 1.416 | 4.000 | 4.000 | 3.802 | 4.000 | 400 | |||
| Notes: | 1. Values > 2.5 are not reliable, they exceed the range of accurate measurement; T/T0 = 0.003. | ||||||||||||
| 2. Group 3 did not wash tissues with mannoside solution before adding TMB/H202 substrate. | |||||||||||||
| 3. Blanks are lost samples. | |||||||||||||