Biotechnology 395, Schedule Number: 140684
Lectures: Tuesday & Thursday 11:30-12:45, McCormack
2nd Floor, Room 415 (M-2-415)
Organizer: Kenneth L. Campbell, M-3-322, Office Hours: T 12:45-2:00
"Biotechnology is the application of knowledge of living systems in
order to use those systems or
their components for industrial purposes." (W. Bains, Biotechnology
from A to Z, 2nd Ed, Oxford
Univ. Press: New York, 1998, 66) This course will follow current
practice and emphasize many
aspects of applied molecular biology, it will include topics covering
the principles and methods of
biotechnology and examples of products in the medical, pharmaceutical,
chemical, agricultural and
environmental industries. The course will be taught by a team
of instructors from the Biology
Department and will include several guest lecturers; a field trip is
also planned.
Prerequisites for the course are permission of the organizer, Biochemistry
383 and 384, and
Molecular Biology 370/372 (corequisites are acceptable). This
year there are two required texts:
Glick and Pasternak, Molecular Biotechnology: Principles and Applications
of Recombinant
DNA, 2nd Ed, ASM Press: Washington, DC, 1998;and, Bains, Biotechnology
from A to Z, 2nd
Ed, Oxford Univ. Press: New York, 1998. In addition, there are
two optional texts available in the
bookstore: Judson, The Eighth Day of Creation: Makers of the Revolution
in Biology,
Expanded Ed, Cold Spring Harbor Press, 1996; and, Kolata, Clone, The
Road to Dolly and the Path Ahead, William Morrow, Inc., 1998. The
readings from the Glick and
Pasternak text are indicated on the lecture schedule. These should
be read before lecture as well as
supplemental readings from papers provided by individual lecturers.
Bain's book is an extended
glossary that should help with material that may not be familiar to
everyone in the class at the time of
the lectures. Other information will be posted to pages associated
with Dr. Campbell's Web Page:
http://kcampbell.bio.umb.edu.
Grading will be based on the results of a take-home mid-term (50%) and
a final (50%). The
mid-term will be handed out about two weeks into the course, will be
based on the first 12 lectures,
and will be due on March 23, 2000. The in-class final, given
during finals week, will be based on
the remaining lectures. The exams will be essays and problems.
There will be no makeup exams
and no extensions for turning in the mid-term. If emergencies
occur contact Dr. Campbell at
287-6676 as soon as possible.
| Biotech Syllabus, B395, Spring Term 2000 | ||||
| Session # | Date | Day | Topic | Speaker |
| 1 | 01/27/00 | Th | Introduction & History of Biotechnology (History of Biotech, G/P 1, J) | Campbell |
| 2 | 02/01/00 | T | Recombinant DNA (Biological Systems, G/P 2) | Kleene |
| 3 | 02/03/00 | Th | Identifying & Isolating Genes (Cloning/Libraries/Transformations, G/P 4) | Kleene |
| 4 | 02/08/00 | T | Gene Expression (RNA/DNA/Protein Synthesis, G/P 3) | Ackerman |
| 5 | 02/10/00 | Th | Prokaryotic Gene Expression (Manipulating Gene Expression in Prokaryotes, G/P 6) | Ackerman |
| 6 | 02/15/00 | T | Oligos & Sequencing (DNA Synthesis & Sequencing, G/P 5) | Kleene |
| 7 | 02/17/00 | Th | Eukaryotic Gene Expression (Gene Expression in Eukaryotes, G/P 7) | Ackerman |
| 8 | 02/22/00 | T | Sequence Searching, ESTs (Nucleic Acid Diagnostics (Blots, PCR), G/P 9) | Kleene |
| 9 | 02/24/00 | Th | Mutagenesis & Protein Engineering (Directed Mutagenesis & Protein Engineering, G/P 8) | Ackerman |
| 10 | 02/29/00 | T | Interaction Trap Screens in Yeast | Gindhart |
| 11 | 03/02/00 | Th | Proteins, Antibiotics, Chemicals (Microbial Production of Therapeutics, G/P 10) | Shiaris |
| 12 | 03/07/00 | T | Bioremediation (Bioremediation, G/P 13) | Shiaris |
| 13 | 03/09/00 | Th | Antibodies & Monoclonal Antibodies (Immunodiagnostics, G/P 9) | Beck |
| 3/11-19/00 | Spring Break | |||
| 14 | 03/21/00 | T | Phage Display (Immunodiagnostics, G/P 9) | Beck |
| 15 | 03/23/00 | Th | Antibodies, Cytokines and Vaccines (Vaccines, G/P 11) & Take-Home Exam Due!!! | Beck |
| 16 | 03/28/00 | T | Protein Purification by Affinity | Sugumaran |
| 17 | 03/30/00 | Th | Rational Drug Design | Sugumaran |
| 03/31/00 | F | P/F, WD deadline | ||
| 18 | 04/04/00 | T | Molecular Pesticides (Microbial Insecticides, G/P 15) | Sugumaran |
| 19 | 04/06/00 | Th | Large Scale Fermentation (Commercial Products by Recombinant Microbes, G/P 12 | Davis |
| & Fermentation Production of Recombinant Products, G/P 16) | ||||
| 20 | 04/11/00 | T | Plant Growth & Nitrogen Fixation (Growth Promotion of Plants by Bacteria, G/P 14) | Davis |
| 21 | 04/13/00 | Th | Plant Transgenesis (Genetic Engineering of Plants, Methods & Applications, G/P 17&18) | Davis |
| 22 | 04/18/00 | T | Genome Projects & Genomics I (Human Molecular Genetics(Mapping & Cloning), G/P 20) | Cook |
| 23 | 04/20/00 | Th | Genome Projects & Genomics II (Human Molecular Genetics(Mapping & Cloning), G/P 20) | Cook |
| 24 | 04/25/00 | T | Animal Transgenics (Transgenesis in Animals, G/P 19) | Campbell |
| 25 | 04/27/00 | Th | Cloning | Campbell |
| 26 | 05/02/00 | T | Gene Therapy (Human Gene Therapy, G/P 21) | Campbell |
| 27 | 05/04/00 | Th | Bioethics (Regulating Use of Biotechnology, G/P 22) | Campbell |
| 28 | 05/09/00 | T | Patent Issues (Patenting Biotechnology, G/P 23) |
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Course Summary & Evaluations | Campbell |