Questions for Biomedical Tracers
Describe the way(s) in which matter may undergo
Define the differences between x-rays and
What is a half life and how is it computed?
What are the usual units of radiation measurement
and how are they interrelated?
What are the usual methods of minimizing radiation
exposure: to particulate radiation; to electromagnetic radiation?
What are the dangers of exposure to radiation,
how do they arise, and is there a root cause for such damage?
What methods are normally employed to quantify
radiation? What instruments are used to measure what types of radioisotope?
What are the major features of the structure
of gamma class immunoglobulins and how might these be used in employing
immunoglobulins as analytical reagents?
What is the process whereby immunoglobulins
are generated by the body? How is this manipulated in vaccination
and in the production of monoclonal antibodies?
What are the major features of haptens and
antigens? What limitations do haptens impose on analytical and localization
Compare and contrast competitive and noncompetitive
What kinds of labels can be used as markers
for competitive immunoassays? For noncompetitive assays?
What are the advantages or disadvantages of
using a kinetic approach versus an endpoint approach to evaluation of enzyme
immunoassay results? Are these opportunities or limits encountered
What are the key features of assay validation
and quality assurance? How are these features measured and evaluated?
Could these approaches be used in other contexts?
Are there any other molecular interaction
systems that share enough major features with antibody-antigen interactions
to allow them to be employed in similar ways in qualitative, quantitative,
and localization studies? Is there a lesson for other tracing methods?
What limitations are involved in using antibodies
to localize antigenic determinants on preserved tissues or in cell cultures?
Can these be overcome? If so, how?
What is the role of standards in immunoanalyses?
How are these generated and/or employed? Is there a lesson for biomedical
tracing in general?