I have put copies of the following articles in the Developmental Lab.

1. Takahashi, Pinto, & Vitaterna, Forward and reverse genetic approaches to behavior in the mouse, Science 264:1724-1733, 1994.
2. Majzoub & Muglia, Molecular Medicine: Knockout mice, NEJM 334:904-907, 1996.
3. Wilmut, Schnieke, McWhir, Kind, & Campbell, Viable offspring derived from fetal and adult mammalian cells, Nature 385:810-813, 1997.
4. Kolata, New questions about popular fertilization technique, NY Times, March 30, 1997.
5. WuDunn, South Korean scientists say they cloned a human cell, NY Times, December 17, 1998.
6. (Special Section) Stem Cell Research and Ethics, Science 287:1417-1446, 2000.

1. Design an approach for generating a mouse which is transgenic beyond the first generation for a single copy gene normally located on the Y chromosome.
2. What are the practical limitations of using animals that are transgenic only in somatic cells?
3. Why are the development of approaches that limit the timing or tissue distribution of expression of transgenes important?
4. Cloning would seem to be a relatively easy thing to do, especially on amphibian eggs. Why have only a few species been successfully cloned?
5. How can transgenesis and cloning be combined?
6. Stem cell development is an exploding area of cellular research and pharmaceutical development. What are its current limitations? How can it be combined with transgenic approaches? And...., why are ethics of any consideration in application of stem cell approaches?