The following Table contains simulated data for the Testosterone Production portion of the Dose-Response Lab.  The first two columns are the information for the assay standards, a single standard curve (from these replicates) should be used to analyze the absorbance readings from the unknowns as given in the last 9 columns.  The information besides the standards is organized by group (or hCG concentration) and time versus tissue originally incubated.  Note that three A450 values are given for each tissue.  Assume this is the value obtained using a 1 to 10 diluted testosterone specimen.    Let me know if there are problems with the data.
 
 

[T] pg/ml	A450	Group	[hCG]	Time	A	A	A	B	B	B	C	C	C
standards					A450a	A450b	A450c	A450a	A450b	A450c	A450a	A450b	A450c
0	0.995	1.000	0.000	0	0.822	0.669	0.974	0.809	0.656	0.962	0.761	0.609	0.916
2	0.920		0.000	5	0.740	0.589	0.894	0.656	0.513	0.807	0.424	0.320	0.539
20	0.787		0.000	20	0.797	0.644	0.950	0.566	0.436	0.706	0.310	0.228	0.401
200	0.488		0.000	60	0.652	0.510	0.802	0.503	0.384	0.633	0.275	0.200	0.357
2000	0.259	2.000	0.04	0	0.766	0.613	0.920	0.708	0.560	0.862	0.744	0.593	0.898
20000	0.146		0.04	5	0.869	0.718	1.015	0.638	0.498	0.787	0.448	0.339	0.568
200000	0.098		0.04	20	0.650	0.508	0.800	0.561	0.433	0.702	0.305	0.224	0.394
2000000	0.055		0.04	60	0.718	0.569	0.872	0.486	0.371	0.614	0.249	0.179	0.326
0	0.974	3.000	0.4	0	0.682	0.536	0.835	0.693	0.546	0.846	0.674	0.530	0.827
2	0.936		0.4	5	0.581	0.449	0.724	0.627	0.488	0.775	0.408	0.307	0.520
20	0.883		0.4	20	0.636	0.496	0.785	0.524	0.402	0.659	0.296	0.217	0.383
200	0.545		0.4	60	0.645	0.504	0.796	0.448	0.339	0.568	0.223	0.157	0.294
2000	0.223	4.000	4	0	0.776	0.623	0.930	0.732	0.582	0.887	0.629	0.490	0.777
20000	0.133		4	5	0.766	0.613	0.920	0.611	0.474	0.757	0.399	0.300	0.509
200000	0.110		4	20	0.658	0.515	0.809	0.483	0.368	0.610	0.291	0.212	0.377
2000000	0.032		4	60	0.615	0.478	0.762	0.426	0.321	0.542	0.220	0.155	0.291
0	1.094	5.000	40	0	0.815	0.662	0.967	0.682	0.536	0.835	0.836	0.683	0.986
2	1.002		40	5	0.708	0.560	0.862	0.579	0.447	0.721	0.431	0.326	0.548
20	0.763		40	20	0.667	0.523	0.819	0.445	0.337	0.564	0.272	0.198	0.354
200	0.486		40	60	0.619	0.481	0.766	0.396	0.298	0.506	0.206	0.143	0.274
2000	0.385	6.000	400	0	0.687	0.541	0.840	0.595	0.461	0.740	0.672	0.528	0.824
20000	0.158		400	5	0.638	0.498	0.787	0.499	0.381	0.629	0.384	0.287	0.491
200000	0.094		400	20	0.591	0.458	0.735	0.418	0.315	0.532	0.262	0.189	0.342
2000000	0.066		400	60	0.744	0.593	0.898	0.341	0.253	0.438	0.193	0.133	0.259